Article
[Expression of mutant horse cytochrome c genes in Escherichia coli].
Bioorganicheskaia khimiia - 1 Oct 1998
Dolgikh D A, Latypov R F, Abdullaev Z Kh, Kolov V, Roder H, Kirpichnikov M P
Abstract excerpt
Here we describe genetically engineered constructs for the expression in Escherichia coli of genes for horse cytochrome c mutants. These constructs allow the expression of the cytochrome c genes together with hemeligase, an enzyme which covalently links heme to cytochrome. Careful selection of producer strains and the adjustment of the conditions of expression provided for expression levels of 10-15 mg of protein...
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