Article
[High expression of human thrombopoietin mutant fusion protein in E. coli].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Dec 1997
Tian S, Liu L, Feng B
Abstract excerpt
OBJECTIVE: To obtain human thrombopoietin (Tpo)mutant cDNA encoding mature peptide N terminal 1 approximately 196 amino acids and investigate its expression in E. coli JM109. METHODS: Polymerase chain reaction and DNA recombination techniques were employed. PCR product was inserted into pUC19 vector and sequenced and then cloned into expression vector pMAL-c2. RESULTS: E. coli JM109 cells with plasmid pMAL...
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