Article
[The use of the multicopy plasmid pUC19 for assuring the constitutive expression of gene rplL in Escherichia coli].
TSitologiia i genetika - 1 Jan 2000
Zolotukhin S B, Zhivolup A N, Krupskaia I V, Budmaska M I, Paton E B
Abstract excerpt
A mutation in lac-operator region of pUC19 plasmid causing an increase in beta-galactosidase activity was observed. The plasmid was used as a vector to provide high level of expression of the cloned E. coli rplL gene.
Topics
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genetic Vectors
- Lac Operon
- Mutation
- Phenotype
- Plasmids
- beta-Galactosidase