Article
Escherichia coli is able to produce heterologous tetraheme cytochrome c(3) when the ccm genes are co-expressed.
Biochimica et biophysica acta - 31 Aug 2000
Herbaud M L, Aubert C, Durand M C, Guerlesquin F, Thöny-Meyer L, Dolla A
Abstract excerpt
The production of Desulfovibrio vulgaris Hildenborough cytochrome c(3) (M(r) 13000), which is a tetraheme cytochrome, in Escherichia coli was examined. This cytochrome was successfully produced in an E. coli strain co-expressing the ccmABCDEFGH genes involved in the cytochrome c maturation process. The apocytochrome c(3) was matured in either anaerobic or aerobic conditions, but aerobic growth in the presence of...
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