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Article

In vitro evolution of horse heart myoglobin to increase peroxidase activity.

Proceedings of the National Academy of Sciences of the United States of America - 27 Oct 1998

Wan L, Twitchett M B, Eltis L D, Mauk A G, Smith M

Abstract excerpt

Random mutagenesis and screening for enzymatic activity has been used to engineer horse heart myoglobin to enhance its intrinsic peroxidase activity. A chemically synthesized gene encoding horse heart myoglobin was subjected to successive cycles of PCR random mutagenesis. The mutated myoglobin gene was expressed in Escherichia coli LE392, and the variants were screened for peroxidase activity with a plate assay....

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 9788999
DOI: 10.1073/pnas.95.22.12825
PMCID: PMC23613

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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