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Article

In vitro evolution of horse heart myoglobin to increase peroxidase activity.

Proceedings of the National Academy of Sciences of the United States of America - 27 Oct 1998

Wan L, Twitchett M B, Eltis L D, Mauk A G, Smith M

Abstract excerpt

Random mutagenesis and screening for enzymatic activity has been used to engineer horse heart myoglobin to enhance its intrinsic peroxidase activity. A chemically synthesized gene encoding horse heart myoglobin was subjected to successive cycles of PCR random mutagenesis. The mutated myoglobin ge...

Topics

Amino Acid Substitution
Animals
Cloning, Molecular
DNA Primers
Directed Molecular Evolution
Escherichia coli
Genetic Variation
Horses
Kinetics
Models, Molecular
Mutagenesis
Myoglobin

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Peroxidases

Point Mutation

Polymerase Chain Reaction

Protein Conformation

Source

Open in PubMed Open full text

Identifiers

PMID: 9788999
DOI: 10.1073/pnas.95.22.12825
PMCID: PMC23613

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9788999-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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