Article
ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.
Genes & development - 15 Jan 1999
Ford L P, Watson J, Keene J D, Wilusz J
Abstract excerpt
We have developed an in vitro mRNA stability system using HeLa cell cytoplasmic S100 extracts and exogenous polyadenylated RNA substrates that reproduces regulated aspects of mRNA decay. The addition of cold poly(A) competitor RNA activated both a sequence-specific deadenylase activity in the ext...
Topics
- Adenosine Triphosphate
- Antigens, Surface
- Base Sequence
- Binding, Competitive
- Cell Extracts
- ELAV Proteins
- ELAV-Like Protein 1
- Genes, Viral
- Genes, fos
- Granulocyte-Macrophage Colony-Stimulating Factor
- HeLa Cells
- Humans
- Kinetics
- Molecular Weight
- Mutation
- Poly A
- Precipitin Tests
- RNA Processing, Post-Transcriptional