Article
An ELISA for RNA helicase activity: application as an assay of the NS3 helicase of hepatitis C virus.
Biochemical and biophysical research communications - 30 Dec 1998
Hsu C C, Hwang L H, Huang Y W, Chi W K, Chu Y D, Chen D S
Abstract excerpt
A convenient enzyme-linked immunosorbent assay (ELISA) for RNA helicase activity was developed with principles similar to the standard assay. The helicase ELISA utilizes a non-radioactive double-stranded substrate with a biotin-labeled template (long) strand hybridized to a digoxigenin (DIG)-labeled release (short) strand. The template strand binds to the wells of streptavidin-coated microtiter plates (SA-MTP)...
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