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Article

The crystal structure of the sevenfold mutant of barley beta-amylase with increased thermostability at 2.5 A resolution.

Journal of molecular biology - 22 Jan 1999

Mikami B, Yoon H J, Yoshigi N

Abstract excerpt

The three-dimensional structure of the sevenfold mutant of barley beta-amylase (BBA-7s) with increased thermostability was determined by X-ray crystallography. The enzyme was purified as a single component and crystallized by a hanging drop method in the presence of 14 % PEG 6000. The crystals belong to space group P43212 with cell dimensions a=b=72.11 A, c=250.51 A. The diffraction data up to 2.5 A were...

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PMID: 9918723
DOI: 10.1006/jmbi.1998.2379

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