Article
Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies.
Proceedings of the National Academy of Sciences of the United States of America - 19 Jan 1999
Xu R, Ayers B, Cowburn D, Muir T W
Abstract excerpt
A convenient in vitro chemical ligation strategy has been developed that allows folded recombinant proteins to be joined together. This strategy permits segmental, selective isotopic labeling of the product. The src homology type 3 and 2 domains (SH3 and SH2) of Abelson protein tyrosine kinase, w...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- Humans
- Magnetic Resonance Spectroscopy
- Mass Spectrometry
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nitrogen Isotopes
- Oncogene Proteins v-abl
- Protein Engineering
- Protein Folding
- Recombinant Fusion Proteins
- src Homology Domains