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Article

Chemical ligation of folded recombinant proteins: segmental isotopic labeling of domains for NMR studies.

Proceedings of the National Academy of Sciences of the United States of America - 19 Jan 1999

Xu R, Ayers B, Cowburn D, Muir T W

Abstract excerpt

A convenient in vitro chemical ligation strategy has been developed that allows folded recombinant proteins to be joined together. This strategy permits segmental, selective isotopic labeling of the product. The src homology type 3 and 2 domains (SH3 and SH2) of Abelson protein tyrosine kinase, which constitute the regulatory apparatus of the protein, were individually prepared in reactive forms that can be...

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Identifiers

PMID: 9892643
DOI: 10.1073/pnas.96.2.388
PMCID: PMC15146

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