Article
Overexpression and biosynthetic deuterium enrichment of TEM-1 beta-lactamase for structural characterization by magnetic resonance methods.
Protein expression and purification - 1 Jul 2000
Sosa-Peinado A, Mustafi D, Makinen M W
Abstract excerpt
An expression system has been developed that allows high levels of production of TEM-1 beta-lactamase with ease of biosynthetic incorporation of nuclear isotopes. The gene for mature TEM-1 beta-lactamase fused to the leader sequence of the ompA protein was subcloned into the pET-24a(+) vector by introduction of an NdeI restriction site at the first codon of the fused genes and transformed into Escherichia coli...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.