Article
An azide-insensitive superoxide dismutase from a hyperthermophilic archaeon, Sulfolobus solfataricus.
Journal of biochemistry - 1 Jan 1999
Yamano S, Maruyama T
Abstract excerpt
The superoxide dismutase (SOD) gene of Sulfolobus solfataricus, a hyperthermophilic archaeon, was cloned and expressed in Escherichia coli, and its gene product was characterized. When the protein was expressed in E. coli, it formed a homodimer that contained both Mn and Fe. Metal reconstitution experiments of the SOD with Fe or Mn showed that only the Fe-reconstituted SOD was active. Substitution of Tyr88 to Phe...
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