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Article

Expression, purification, and characterization of recombinant S-adenosylhomocysteine hydrolase from the thermophilic archaeon Sulfolobus solfataricus.

Protein expression and purification - 1 Feb 2000

Porcelli M, Fusco S, Inizio T, Zappia V, Cacciapuoti G

Abstract excerpt

S-Adenosylhomocysteine hydrolase from Sulfolobus solfataricus was expressed in Escherichia coli by inserting the genomic fragment containing the gene encoding for S-adenosylhomocysteine hydrolase downstream the isopropyl-beta-d-thiogalactoside-inducible promoter of pTrc99A expression vector. An ATG positioned 25 bp upstream of the gene which is in frame with a stop codon was utilized as the initiation codon. This...

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PMID: 10648166
DOI: 10.1006/prep.1999.1161

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SDS

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