Article
Zinc-coordination of aspartic acid-76 in Sulfolobus ferredoxin is not required for thermal stability of the molecule.
Journal of inorganic biochemistry - 10 Apr 2002
Kojoh Kanehisa, Fukuda Eriko, Matsuzawa Hiroshi, Wakagi Takayoshi
Abstract excerpt
The highly thermostable 7Fe-ferredoxin from Sulfolobus sp. strain 7 has tightly bound zinc at the interface between the N-terminal extra domain and the C-terminal core. The zinc is tetrahedrally ligated by His-16, His-19, His-34, and Asp-76. Previous studies on truncated mutants have shown that the zinc and certain parts, i.e. not all, of the N-terminal extra stretch are responsible for the thermal stabilization...
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