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Article

Effects of C-terminal deletion on the activity and thermostability of orotate phosphoribosyltransferase from Thermus thermophilus.

Journal of biochemistry - 1 Jan 1999

Hamana H, Shinozawa T

Abstract excerpt

To investigate the role of the C-terminal region on the activity and thermostability of orotate phosphoribosyltransferase (OPRTase, EC 2. 4.2.10) from Thermus thermophilus, four C-terminal amino acid-deleted OPRTases (1, 2, 3, and 5 residues deleted) were constructed. The activities of all the mu...

Topics

Enzyme Stability
Hot Temperature
Kinetics
Mutation
Orotate Phosphoribosyltransferase
Orotic Acid
Phosphoribosyl Pyrophosphate
Protein Denaturation
Recombinant Proteins
Sequence Deletion
Temperature
Thermus thermophilus

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Identifiers

PMID: 9880805
DOI: 10.1093/oxfordjournals.jbchem.a022246

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9880805-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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