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[Cloning, expression and characterization of the hypoxanthine-guanine phosphoribosyltransferase mutants from T. tengcongensis].

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica - 1 Sept 2003

You De-Lin, Qu Hong, Chen Qiang, Xing Yang, Gu Xiao-Cheng, Luo Ming

Abstract excerpt

Based on a predicted three-dimensional structure of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) from Thermoanaerobacter tengcongensis, three mutants of HGPRT were designed to modify the purine specificity of HGPRT. Site-directed mutagenesis was used to generate the three mutants, K(133)A, K(133)S, K(133)T. Wild type HGPRT and its mutants were expressed E. coli in BL21 (DE3) pLysS, and the expression...

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PMID: 12958660

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