Article
Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae.
Current genetics - 1 Feb 1990
de Montigny J, Kern L, Hubert J C, Lacroute F
Abstract excerpt
Orotate phosphoribosyl transferase (OP-RTase) catalyses the transformation of orotate to OMP in the pyrimidine pathway. In the yeast Saccharomyces cerevisiae, the URA5 gene is known to encode this enzyme activity. In this paper we present the cloning and sequencing of a yeast gene, named URA10, encoding a second OPRTase enzyme. Comparison of the predicted amino acid sequences between URA5 and URA10 genes shows...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cloning, Molecular
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Orotate Phosphoribosyltransferase
- Pentosyltransferases
- Phenotype
- Pyrimidines