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Article

Differential epitope tagging of actin in transformed Drosophila produces distinct effects on myofibril assembly and function of the indirect flight muscle.

Molecular biology of the cell - 1 Jan 1999

Brault V, Sauder U, Reedy M C, Aebi U, Schoenenberger C A

Abstract excerpt

We have tested the impact of tags on the structure and function of indirect flight muscle (IFM)-specific Act88F actin by transforming mutant Drosophila melanogaster, which do not express endogenous actin in their IFMs, with tagged Act88F constructs. Epitope tagging is often the method of choice to monitor the fate of a protein when a specific antibody is not available. Studies addressing the functional...

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Identifiers

PMID: 9880332
DOI: 10.1091/mbc.10.1.135
PMCID: PMC25159

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