Article
Threonine overproduction in yeast strains carrying the HOM3-R2 mutant allele under the control of different inducible promoters.
Applied and environmental microbiology - 1 Jan 1999
Farfán M J, Aparicio L, Calderón I L
Abstract excerpt
The HOM3 gene of Saccharomyces cerevisiae codes for aspartate kinase, which plays a crucial role in the regulation of the metabolic flux that leads to threonine biosynthesis. With the aim of obtaining yeast strains able to overproduce threonine in a controlled way, we have placed the HOM3-R2 muta...
Topics
- Alleles
- Aspartate Kinase
- Base Sequence
- DNA, Fungal
- Gene Expression
- Genes, Fungal
- Mutagenesis, Site-Directed
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Saccharomyces cerevisiae
- Threonine
- Transformation, Genetic