Article
Diastereoselective synthesis of L: -threo-3,4-dihydroxyphenylserine by low-specific L: -threonine aldolase mutants.
Biotechnology letters - 1 Jan 2010
Gwon Hui-Jeong, Baik Sang-Ho
Abstract excerpt
Diastereoselectivity-enhanced mutants of L: -threonine aldolase (L: -TA) for L: -threo-3,4-dihydroxyphenylserine (L: -threo-DOPS) synthesis were isolated by error-prone PCR followed by a high-throughput screening. The most improved mutant was achieved from the mutant T3-3mm2, showing a 4-fold increase over the wild-type L: -TA. When aldol condensation activity was examined using whole cells of T3-3mm2, its de was...
Topics
- Bacterial Proteins
- Droxidopa
- Escherichia coli
- Glycine Hydroxymethyltransferase
- Molecular Structure
- Mutagenesis, Site-Directed
- Mutation
- Polymerase Chain Reaction
- Streptomyces coelicolor