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[Construction of recombinant plasmids containing threonine operon and their effects on L-threonine accumulation].

Wei sheng wu xue bao = Acta microbiologica Sinica - 1 May 2009

Zhang Xue, Yan Ji'ai, Yu Lei, Zhang Guoqiang, Zhang Yun, Chen Ning, Wen Tingyi

Abstract excerpt

OBJECTIVE: We reconstructed two recombinant plasmids and studied their effects on L-threonine accumulation of Escherichia coli W3110. METHODS: We amplified the threonine operon containing ThrLp promoter, lead peptide thrL, thrA, thrB and thrC genes by PCR from E. coli W3110 chromosome and ligated it into the pMD19 T-vector. Site-directed mutation were carried out by gene splicing by overlap extension PCR to...

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PMID: 19637565

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