Article
PCR cloning and detection of point mutations in the eburicol 14alpha-demethylase (CYP51) gene from Erysiphe graminis f. sp. hordei, a "recalcitrant" fungus.
Current genetics - 1 Dec 1998
Délye C, Bousset L, Corio-Costet M F
Abstract excerpt
Molecular studies of some micro-organisms are hampered by the difficulty of obtaining sufficient amounts of nucleic acids. A cloning strategy based on PCR has therefore been used to clone the eburicol 14alpha-demethylase (CYP51) gene of the obligate fungus Erysiphe graminis f. sp. hordei (Egh) us...
Topics
- Alleles
- Amino Acid Sequence
- Ascomycota
- Cloning, Molecular
- Cytochrome P-450 Enzyme System
- DNA, Complementary
- DNA, Fungal
- Gene Amplification
- Genes, Fungal
- Genetic Variation
- Molecular Sequence Data
- Oxidoreductases
- Point Mutation
- Polymerase Chain Reaction
- Sequence Alignment
- Sequence Homology, Amino Acid
- Sterol 14-Demethylase