Article
RNA polymerase subunit RPB5 plays a role in transcriptional activation.
Proceedings of the National Academy of Sciences of the United States of America - 22 Dec 1998
Miyao T, Woychik N A
Abstract excerpt
A mutation in RPB5 (rpb5-9), an essential RNA polymerase subunit assembled into RNA polymerases I, II, and III, revealed a role for this subunit in transcriptional activation. Activation by GAL4-VP16 was impaired upon in vitro transcription with mutant whole-cell extracts. In vivo experiments usi...
Topics
- Conserved Sequence
- Fungal Proteins
- Genotype
- Humans
- Kinetics
- Macromolecular Substances
- Mutagenesis, Site-Directed
- Plasmids
- Polymerase Chain Reaction
- Promoter Regions, Genetic
- RNA Polymerase I
- RNA Polymerase II
- RNA Polymerase III
- Recombinant Fusion Proteins
- Saccharomyces cerevisiae
- Trans-Activators
- Transcription, Genetic
- Transcriptional Activation