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Article

The C331A mutant of neuronal nitric-oxide synthase is defective in arginine binding.

The Journal of biological chemistry - 25 Dec 1998

Martásek P, Miller R T, Liu Q, Roman L J, Salerno J C, Migita C T, Raman C S, Gross S S, Ikeda-Saito M, Masters B S

Abstract excerpt

It has been proposed that Cys99 of human endothelial nitric oxide synthase (eNOS) is responsible for tetrahydrobiopterin (BH4) binding. To examine this possibility rigorously, we expressed rat neuronal NOS (nNOS) in Escherichia coli, with the homologous Cys331 to Ala mutation, and characterized structural and functional attributes of the purified, mutated enzyme. C331A-nNOS, as isolated, was catalytically...

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PMID: 9857005
DOI: 10.1074/jbc.273.52.34799

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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