Article
Circular dichroism study of ribonuclease A mutants containing the minimal structural requirements for dimerization and swapping.
International journal of biological macromolecules - 1 Nov 1998
Catanzano F, Graziano G, Cafaro V, D'Alessio G, Di Donato A, Barone G
Abstract excerpt
Four residues Pro19. Leu28, Cys31 and Cys32 proved to be the minimal structural requirements in determining the dimeric structure and the N-terminal segment swapping of bovine seminal ribonuclease, BS-RNase. We analyzed the content of secondary and tertiary structures in RNase A, P-RNase A, PL-RN...
Topics
- Animals
- Cattle
- Circular Dichroism
- Dimerization
- Enzyme Stability
- Hydrogen-Ion Concentration
- Mutation
- Protein Conformation
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Ribonuclease, Pancreatic
- Thermodynamics