Article
Generation of CMRF-44+ monocyte-derived dendritic cells: insights into phenotype and function.
Experimental hematology - 1 Dec 1998
Vuckovic S, Fearnley D B, Mannering S I, Dekker J, Whyte L F, Hart D N
Abstract excerpt
The CMRF-44 monoclonal antibody (MoAb) recognizes an intermediate stage of blood dendritic cell (DC) differentiation as well as mature CD83+ blood DC. Here we describe the use of the CMRF-44 MoAb to monitor the in vitro development of DC-like cells from peripheral blood mononuclear cells. Neither granulocyte-macrophage colony-stimulating factor (GM-CSF) nor GM-CSF plus tumor necrosis factor-alpha (TNF-alpha)...
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