Article
Unilineage monocytopoiesis in hematopoietic progenitor culture: switching cytokine treatment at all Mo developmental stages induces differentiation into dendritic cells.
Cell death and differentiation - 1 Feb 2006
Montesoro E, Castelli G, Morsilli O, Nisini R, Stafsnes M H, Carè A, Peschle C, Chelucci C
Abstract excerpt
We have developed a new culture system whereby human hematopoietic progenitors purified from adult peripheral blood extensively proliferate and gradually differentiate into >95% pure monocytic (Mo) cells. At all developmental stages treatment with interleukin (IL)-4+granulocyte-macrophage colony-stimulating factor or IL-4+c-Kit-ligand+FLT-3 ligand switched the Mo precursors into dendritic cells (DCs). The...
Topics
- Cell Lineage
- Cell Proliferation
- Cells, Cultured
- Cytokines
- Dendritic Cells
- Gene Expression Regulation
- Gene Transfer Techniques
- Genetic Vectors
- Granulocyte-Macrophage Colony-Stimulating Factor
- Hematopoietic Stem Cells
- Humans
- Immunotherapy