Attenuation of drug-stimulated topoisomerase II-DNA cleavable complex formation in wild-type HL-60 cells treated with an intracellular calcium buffer is correlated with decreased cytotoxicity and site-specific hypophosphorylation of topoisomerase IIalpha.

doi:10.1042/bj3360727

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Article

Attenuation of drug-stimulated topoisomerase II-DNA cleavable complex formation in wild-type HL-60 cells treated with an intracellular calcium buffer is correlated with decreased cytotoxicity and site-specific hypophosphorylation of topoisomerase IIalpha.

The Biochemical journal - 15 Dec 1998

Aoyama M, Grabowski D R, Dubyak G R, Constantinou A I, Rybicki L A, Bukowski R M, Ganapathi M K, Hickson I D, Ganapathi R

Abstract excerpt

Topoisomerase II (topo II), an essential enzyme for cell viability, is also the target for clinically important anti-neoplastic agents that stimulate topo II-mediated DNA scission. The role of alterations in topo IIalpha phosphorylation and its effect on drug-induced DNA damage and cytotoxicity w...

Topics

Antigens, Neoplasm
Antineoplastic Agents
Buffers
Calcium
Chelating Agents
DNA
DNA Topoisomerases, Type II
DNA-Binding Proteins
Doxorubicin
Egtazic Acid

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Ethylenediamines

HL-60 Cells

Humans

Isoenzymes

Peptide Mapping

Phenotype

Phosphorylation

Source

Open in PubMed Open full text

Identifiers

PMID: 9841887
DOI: 10.1042/bj3360727
PMCID: PMC1219926

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9841887-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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