Article
A temperature sensitive topoisomerase II allele confers temperature dependent drug resistance on amsacrine and etoposide: a genetic system for determining the targets of topoisomerase II inhibitors.
Cancer research - 1 Jan 1993
Nitiss J L, Liu Y X, Hsiung Y
Abstract excerpt
We have developed a simple system for determining the specific contribution of topoisomerase II targeting to the cytotoxic activity of a drug. We have constructed yeast strains that are permeable to anti-topoisomerase II drugs, carry a DNA repair mutation, rad52, and also have a temperature sensi...
Topics
- Alleles
- Amsacrine
- Camptothecin
- Cell Division
- DNA Damage
- DNA Repair
- DNA Topoisomerases, Type II
- Drug Resistance
- Etoposide
- Models, Biological
- Saccharomyces cerevisiae
- Sensitivity and Specificity
- Temperature
- Topoisomerase II Inhibitors