Article
Mechanistic studies of amsacrine-resistant derivatives of DNA topoisomerase II. Implications in resistance to multiple antitumor drugs targeting the enzyme.
The Journal of biological chemistry - 19 Aug 1994
Wasserman R A, Wang J C
Abstract excerpt
Wild-type yeast DNA topoisomerase II and three of its amsacrine-resistant derivatives L475A/L480P, L475A/R476G, and A642G, named according to amino acid changes at the codons specified, were overexpressed and purified. Because cells expressing several mutant enzymes missing portions of the carboxyl-terminal domain of the wild-type enzyme were previously found to exhibit amsacrine resistance, a carboxyl-terminal...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Amsacrine
- Biological Transport
- DNA
- DNA Topoisomerases, Type II
- Drug Resistance
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae