Article
Oligosaccharides of recombinant mouse gelatinase B variants.
Biochimica et biophysica acta - 27 Nov 1998
Van den Steen P, Rudd P M, Proost P, Martens E, Paemen L, Küster B, van Damme J, Dwek R A, Opdenakker G
Abstract excerpt
Gelatinase B (matrix metalloproteinase-9, MMP-9) contains three N-glycosylation sites and a Ser/Thr/Pro-rich type V collagen domain with repetitive attachment sites for O-linked sugars. Recombinant mouse gelatinase B was expressed in the yeast Pichia pastoris and the N-linked oligosaccharides of the truncated glycoprotein variants were analysed by in gel enzymatic release followed by mass spectrometry and normal...
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