Article
Oligosaccharides of recombinant mouse gelatinase B variants.
Biochimica et biophysica acta - 27 Nov 1998
Van den Steen P, Rudd P M, Proost P, Martens E, Paemen L, Küster B, van Damme J, Dwek R A, Opdenakker G
Abstract excerpt
Gelatinase B (matrix metalloproteinase-9, MMP-9) contains three N-glycosylation sites and a Ser/Thr/Pro-rich type V collagen domain with repetitive attachment sites for O-linked sugars. Recombinant mouse gelatinase B was expressed in the yeast Pichia pastoris and the N-linked oligosaccharides of...
Topics
- Acetaldehyde
- Animals
- Chromatography, High Pressure Liquid
- Collagenases
- Glycoside Hydrolases
- Hemopexin
- Matrix Metalloproteinase 9
- Mice
- Mutation
- Oligosaccharides
- Pichia
- Polysaccharides
- Recombinant Proteins
- Sequence Analysis