Article
Virological importance of the protease-cleavage site in human immunodeficiency virus type 1 Nef is independent of both intravirion processing and CD4 down-regulation.
Virology - 25 Nov 1998
Pandori M, Craig H, Moutouh L, Corbeil J, Guatelli J
Abstract excerpt
The HIV-1 Nef protein is present within the virion and is processed there by the viral protease. Mutational analysis indicated that residues 54-60 in HIV-1 Nef were required for intravirion cleavage. When viruses were produced using T cell lines or primary lymphoblasts, these residues were also r...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- CD4 Antigens
- DNA, Viral
- Deoxyribonucleases, Type II Site-Specific
- Down-Regulation
- Gene Products, nef
- HIV-1
- HeLa Cells
- Humans
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Phenotype
- Structure-Activity Relationship
- Transfection
- Virion
- nef Gene Products, Human Immunodeficiency Virus