Article
Analysis of polymerase chain reaction products by high-performance liquid chromatography with fluorimetric detection and its application to DNA diagnosis.
Journal of chromatography. B, Biomedical sciences and applications - 25 Sept 1998
Arakawa H, Nakashiro S, Tsuji A, Maeda M
Abstract excerpt
We describe the development of a sensitive high-performance liquid chromatographic (HPLC) method for polymerase chain reaction (PCR) products using bisbenzimide (Hoechst 33258 dye) based fluorimetric detection. The detection limit and specificity for double-strand DNA detection are improved in co...
Topics
- Alleles
- Bacteriophage phi X 174
- Chromatography, High Pressure Liquid
- DNA Mutational Analysis
- DNA Probes
- DNA, Viral
- Fluorescein-5-isothiocyanate
- Fluorescent Dyes
- Fluorometry
- Genes, ras
- Humans
- Infant, Newborn
- Neonatal Screening
- Nucleic Acid Hybridization
- Phenylketonurias
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length