Article
An assay for O6-alkylguanine-DNA alkyltransferase based on restriction endonuclease inhibition and magnetic bead separation of products.
Carcinogenesis - 1 Oct 1994
Wilson B D, Strauss M, Stickells B J, Hoal-van Helden E G, van Helden P
Abstract excerpt
We describe an improved, rapid and cost effective assay for measuring O6-alkylguanine-DNA alkyltransferase (AGT) levels in large numbers of small biological samples. The assay is based on the use of a synthetic O6-methylguanine oligonucleotide bound to magnetic beads via a biotin-streptavidin linkage and bound to its complement. A 35S label is incorporated into the free end of the duplex. The basis of the assay...
Topics
- Base Sequence
- DNA
- Deoxyribonucleases, Type II Site-Specific
- Escherichia coli
- Feasibility Studies
- Humans
- Hydrolysis
- Immunomagnetic Separation
- Lymphocytes
- Methyltransferases
- Molecular Sequence Data