A comparison of calcium phosphate coprecipitation and electroporation. Implications for studies on the genetic effects of DNA damage.

doi:10.1007/BF02760857

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Article

A comparison of calcium phosphate coprecipitation and electroporation. Implications for studies on the genetic effects of DNA damage.

Molecular biotechnology - 1 Oct 1998

Nickoloff J A, Spirio L N, Reynolds R J

Abstract excerpt

Plasmid-based transfection assays provide a rapid means to measure homologous and nonhomologous recombination in mammalian cells. Often it is of interest to examine the stimulation of recombination by DNA damage induced by radiation, genotoxic chemicals, or nucleases. Transfection is frequently p...

Topics

Animals
CHO Cells
Calcium Phosphates
Cricetinae
DNA
DNA Damage
DNA, Circular
Electroporation
Genetic Vectors
Mutation
Plasmids
Recombination, Genetic
Transfection

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