Article
A comparison of calcium phosphate coprecipitation and electroporation. Implications for studies on the genetic effects of DNA damage.
Molecular biotechnology - 1 Oct 1998
Nickoloff J A, Spirio L N, Reynolds R J
Abstract excerpt
Plasmid-based transfection assays provide a rapid means to measure homologous and nonhomologous recombination in mammalian cells. Often it is of interest to examine the stimulation of recombination by DNA damage induced by radiation, genotoxic chemicals, or nucleases. Transfection is frequently performed by using calcium phosphate coprecipitation (CPP), because this method is well suited for handling large sample...
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