Article
Electroporation of Haemophilus influenzae is effective for transformation of plasmid but not chromosomal DNA.
Nucleic acids research - 11 Jul 1991
Mitchell M A, Skowronek K, Kauc L, Goodgal S H
Abstract excerpt
Electroporation of plasmid and chromosomal DNAs were tested in Haemophilus influenzae because of an interest in introducing DNA into mutants that are deficient in competence for transformation. The initial experiments were designed to investigate and optimize conditions for electroporation of H. influenzae. Plasmid DNA was introduced into the competence proficient strain Rd and its competence-deficient uptake...
Topics
- Ampicillin Resistance
- Base Sequence
- Chromosomes, Bacterial
- DNA, Bacterial
- Deoxyribonuclease I
- Electric Stimulation
- Genetic Markers
- Haemophilus influenzae
- Molecular Sequence Data
- Mutation
- Plasmids