Article
Pathway of complex formation between DNA and three subunits of CBF/NF-Y. Photocross-linking analysis of DNA-protein interaction and characterization of equilibrium steps of subunit interaction and dna binding.
The Journal of biological chemistry - 20 Nov 1998
Liang S G, Maity S N
Abstract excerpt
In this study, we used a photocross-linking method to identify specific contact of CCAAT-binding factor (CBF) subunits in a CBF-DNA complex. The analysis showed that all three subunits in the CBF-DNA complex were cross-linked to DNA and that CBF-B and CBF-C were cross-linked more strongly than CB...
Topics
- Bromodeoxyuridine
- CCAAT-Enhancer-Binding Proteins
- Cross-Linking Reagents
- DNA
- DNA-Binding Proteins
- Dimerization
- Models, Chemical
- Mutation
- Photochemistry
- Protein Binding
- Protein Conformation
- Surface Properties
- Ultraviolet Rays