Article
In vivo assay of protein-protein interactions in Hin-mediated DNA inversion.
Journal of bacteriology - 1 Nov 1998
Lee S Y, Lee H J, Lee H, Kim S, Cho E H, Lim H M
Abstract excerpt
In order to form the catalytic nucleoprotein complex called the invertasome in the Hin-mediated DNA inversion reaction, interactions of the DNA-binding proteins Hin and Fis are required. Assays for these protein-protein interactions have been exploited with protein cross-linkers in vitro. In this study, an in vivo assay system that probes protein-protein interactions was developed. The formation of a DNA loop...
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