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Article

Evaluation of a Novel Mononuclear Cell Isolation Procedure for Serological HLA Typing

1 Nov 1998

Abstract excerpt

Despite recent advances in DNA-based genotyping, the microcytotoxicity test is still broadly used for the determination of human leukocyte class I antigens in patients as well as organ donors and also for the detection of HLA antibodies. Excellent purity and viability of peripheral blood mononuclear cells (PBMC) are essential for reliable HLA typing results. Background staining and cell loss can contribute to...

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PMID: 9801339
DOI: 10.1128/cdli.5.6.808-813.1998

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