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High-throughput detection and multiplex identification of cell contaminations

9 Jul 2009

Abstract excerpt

Unnoticed cell culture contamination by viruses, Mycoplasma, or other cell lines is not uncommon and a threat to laboratory safety and the quality of scientific results. We developed and validated a novel high-throughput Multiplex cell Contamination Test (McCT), which is currently able to detect 37 contamination markers in a single reaction. The assay is based on multiplex PCR with target-specific primers and...

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PMID: 19589807
DOI: 10.1093/nar/gkp581

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