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Isolation, Cryopreservation, and Immunophenotyping of Human Peripheral Blood Mononuclear Cells

1 Nov 2017

Abstract excerpt

This unit describes procedures for the isolation, cryopreservation, and thawing of human peripheral blood mononuclear cells (HPBMC) and analysis of cell surface markers (CSM) for immunophenotyping using polychromatic flow cytometry. This methodology can be used to ensure that cell integrity and phenotype stability are not altered through cryopreservation and extended storage. For this analysis, HPBMC were...

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PMID: 29117436
DOI: 10.1002/cptx.31

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