Article
HLA-targeted flow cytometric sorting of blood cells allows separation of pure and viable microchimeric cell populations.
Blood - 10 Nov 2011
Drabbels Jos J M, van de Keur Carin, Kemps Berit M, Mulder Arend, Scherjon Sicco A, Claas Frans H J, Eikmans Michael
Abstract excerpt
Microchimerism is defined by the presence of low levels of nonhost cells in a person. We developed a reliable method for separating viable microchimeric cells from the host environment. For flow cytometric cell sorting, HLA antigens were targeted with human monoclonal HLA antibodies (mAbs). Optimal separation of microchimeric cells (present at a proportion as low as 0.01% in artificial mixtures) was obtained with...
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