Article
Denaturing gradient gel electrophoresis: a rapid method for differentiating BoLA-DRB3 alleles.
Animal genetics - 1 Oct 1998
Aldridge B M, McGuirk S M, Clark R J, Knapp L A, Watkins D I, Lunn D P
Abstract excerpt
The products of the BoLA-DRB3 locus are important molecules in the bovine immune response. Several techniques have been used to study and define this locus but they are generally time consuming and limited in their ability to detect novel alleles. In this study we used denaturing gradient gel electrophoresis (DGGE), and direct sequencing, for BoLA-DRB3-typing. First, modified locus-specific primers were used in...
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