Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos indicus) by temperature-gradient gel electrophoresis (TGGE) and direct sequencing.

doi:10.1111/j.1365-2370.2004.00438.x

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Article

Genotyping BoLA-DRB3 alleles in Brazilian Dairy Gir cattle (Bos indicus) by temperature-gradient gel electrophoresis (TGGE) and direct sequencing.

European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Feb 2004

Da Mota A F, Martinez M L, Coutinho L L

Abstract excerpt

BoLA-DRB3 is a gene of the major histocompatibility complex (MHC) in cattle. The product of the BoLA-DRB3 gene is a beta chain of an MHC class II molecule, a glycoprotein expressed on the surface of antigen-presenting cells (APCs). Responses of CD4+ T lymphocytes to peptides are dependent on the presentation of peptide ligands bound to class II molecules on APCs. Genotyping of the BoLA-DRB3 gene is relatively...

Topics

Alleles
Animals
Antigen-Presenting Cells
CD4-Positive T-Lymphocytes
Cattle
Cloning, Molecular
DNA
Electrophoresis
Exons
Genotype
Glycoproteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Heterozygote

Histocompatibility Antigens Class II

Humans

Peptides

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Sequence Analysis, DNA

Source

Open in PubMed

Identifiers

PMID: 15009179
DOI: 10.1111/j.1365-2370.2004.00438.x

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