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Article

Multi-primer target PCR for rapid identification of bovine DRB3 alleles.

Animal genetics - 1 Aug 2001

Ledwidge S A, Mallard B A, Gibson J P, Jansen G B, Jiang Z H

Abstract excerpt

Multi-primer target polymerase chain reaction (MPT-PCR) is a rapid method for the identification of specific BoLA-DRB3 alleles. In a single PCR reaction, the presence of two alleles associated with increased risk, DRB3.2*23 (DRB3*2701-2703, 2705-2707) and decreased risk, DRB3.2*16 (DRB3*1501, 1502), of mastitis in Canadian Holstein can be detected. Two outer primers amplify exon 2 of DRB3. Simultaneously, two...

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PMID: 11531702
DOI: 10.1046/j.1365-2052.2001.00761.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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