Article
Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP.
Animal genetics - 1 Jan 1992
van Eijk M J, Stewart-Haynes J A, Lewin H A
Abstract excerpt
A polymerase chain reaction (PCR)-based method is described for typing of alleles of the bovine lymphocyte antigen (BoLA)-DRB3 gene. A total of 30 DRB3 alleles were distinguished by digestion of PCR amplification products of BoLA-DRB3 exon 2 with RsaI, BstYI and HaeIII (PCR-RFLP). All restriction fragment patterns, with the exception of one HaeIII pattern, were consistent with restriction sites that were found...
Topics
- Alleles
- Animals
- Base Sequence
- Cattle
- DNA
- Exons
- Genes, MHC Class II
- Haplotypes
- Major Histocompatibility Complex
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length