Article
Identification of in vivo substrates of the yeast mitochondrial chaperonins reveals overlapping but non-identical requirement for hsp60 and hsp10.
The EMBO journal - 15 Oct 1998
Dubaquié Y, Looser R, Fünfschilling U, Jenö P, Rospert S
Abstract excerpt
The mechanism of chaperonin-assisted protein folding has been mostly analyzed in vitro using non-homologous substrate proteins. In order to understand the relative importance of hsp60 and hsp10 in the living cell, homologous substrate proteins need to be identified and analyzed. We have devised a...
Topics
- Chaperonin 10
- Chaperonin 60
- Chaperonins
- Electrophoresis, Gel, Two-Dimensional
- Mitochondria
- Mutation
- Protein Biosynthesis
- Protein Folding
- Protein Precursors
- RNA, Messenger
- Saccharomyces cerevisiae
- Substrate Specificity
- Temperature
- Thiosulfate Sulfurtransferase