Article
A targeted DNA-PKcs-null mutation reveals DNA-PK-independent functions for KU in V(D)J recombination.
Immunity - 1 Sept 1998
Gao Y, Chaudhuri J, Zhu C, Davidson L, Weaver D T, Alt F W
Abstract excerpt
The DNA-dependent protein kinase (DNA-PK) consists of Ku70, Ku80, and a large catalytic subunit, DNA-PKcs. Targeted inactivation of the Ku70 or Ku80 genes results in elevated ionizing radiation (IR) sensitivity and inability to perform both V(D)J coding-end and signal (RS)-end joining in cells, w...
Topics
- Animals
- Antigens, Nuclear
- DNA Helicases
- DNA-Activated Protein Kinase
- DNA-Binding Proteins
- Embryo, Mammalian
- Female
- Fibroblasts
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Gene Targeting
- Immunoglobulin Joining Region
- Ku Autoantigen
- Male
- Mice
- Mice, Inbred C57BL
- Mice, Knockout
- Mutation
- Nuclear Proteins