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Article

Generation of Infectious Pancreatic Necrosis Virus from Cloned cDNA

1 Nov 1998

Abstract excerpt

We developed a reverse genetics system for infectious pancreatic necrosis virus (IPNV), a prototype virus of the Birnaviridae family, with the use of plus-stranded RNA transcripts derived from cloned cDNA. Full-length cDNA clones of the IPNV genome that contained the entire coding and noncoding regions of RNA segments A and B were constructed. Segment A encodes a 106-kDa precursor protein which is cleaved to...

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PMID: 9765436
DOI: 10.1128/jvi.72.11.8913-8920.1998

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