Article
Effects of mutations in the gamma-phosphate binding site of myosin on its motor function.
The Journal of biological chemistry - 16 Oct 1998
Li X D, Rhodes T E, Ikebe R, Kambara T, White H D, Ikebe M
Abstract excerpt
The role of the highly conserved residues in the gamma-phosphate binding site of myosin upon myosin motor function was studied. Each of five residues (Ser181, Lys185, Asn235, Ser236, and Arg238) in smooth muscle myosin was mutated. K185Q has neither a steady state ATPase nor an initial Pi burst....
Topics
- Actins
- Adenosine Diphosphate
- Adenosine Triphosphate
- Binding Sites
- Catalytic Domain
- Flow Injection Analysis
- Hydrolysis
- Models, Chemical
- Models, Molecular
- Motion
- Muscle, Smooth
- Mutation
- Myosins
- Protein Binding
- Recombinant Proteins