Article
A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast.
Proceedings of the National Academy of Sciences of the United States of America - 13 Dec 2005
Proszynski Tomasz J, Klemm Robin W, Gravert Maike, Hsu Peggy P, Gloor Yvonne, Wagner Jan, Kozak Karol, Grabner Hannes, Walzer Karen, Bagnat Michel, Simons Kai, Walch-Solimena Christiane
Abstract excerpt
Recently synthesized proteins are sorted at the trans-Golgi network into specialized routes for exocytosis. Surprisingly little is known about the underlying molecular machinery. Here, we present a visual screen to search for proteins involved in cargo sorting and vesicle formation. We expressed a GFP-tagged plasma membrane protein in the yeast deletion library and identified mutants with altered marker...
Topics
- Biological Transport
- Calcium-Binding Proteins
- DNA Mutational Analysis
- Ergosterol
- Gene Library
- Genes, Fungal
- Green Fluorescent Proteins
- Intracellular Signaling Peptides and Proteins
- Membrane Glycoproteins